![]() In one embodiment, single copies of the nucleic acid template species are hybridized to capture beads comprising, e.g., capture oligonucleotides or chemical groups that bind to the nucleic acid template. This invention further provides means for encapsulating a plurality of DNA samples individually in a microcapsule of an emulsion (i.e., a microreactor), performing amplification of the plurality of encapsulated nucleic acid samples simultaneously, and releasing said amplified plurality of DNA from the microcapsules for subsequent reactions. One use of the method of the invention is to perform simultaneous clonal amplification (e.g., by PCR) of a plurality of samples (as many as several hundred thousand) in one reaction vessel. The present invention provides for a method of amplifying a plurality of nucleic acids (e.g., each sequence of a DNA library, transcriptome, or genome) in a rapid and economical manner in a single reaction tube. Individual amplification of each fragment of these libraries in separate reaction is not practical. However, this approach may be impractical if many thousands of separate reaction tubes are required for the amplification process, as a genomic library or cDNA library may include more than 100,000 fragments. This problem with PCR may be overcome if each individual member of a library is amplified in a separate reaction. That is, in a random PCR environment, some DNA sequences are preferentially amplified at the expense of other sequences such that the amplified product does not represent the starting material. While random primed PCR can be easily engineered to amplify a plurality of nucleic acids in one reaction, this method is not preferred because the amplified library is not representative of the starting library. Other techniques, such as PCR, while fast and reliable, are unable to amplify a genome in a representative fashion. Current techniques for in vitro genome amplification involve laborious cloning and culturing protocols that have limited the utility of genomic sequencing. The starting material may be limited, for example, if the genome to be sequenced is from a trace quantity of pathogen or from a prenatal patient. If the starting material is limited, amplification of the initial DNA is necessary before genomic sequencing. Furthermore, the sequencing of a human genome would require about tens of millions of different sequencing reactions. Current sequencing technologies require millions of copies of nucleic acid per sequencing reaction. The ability to amplify a plurality of nucleic acid sequences, such as a genomic library or a cDNA library, is critical given the inefficiency of current methods of sequencing. The present invention is also directed to zero bead removal-a method of enriching for solid support containing amplified nucleic acids is also disclosed. The present invention relates to methods for amplifying nucleic acid templates from low copy number to quantities amenable for sequencing on a solid support such as a bead. 16, 2019 and is 6 KB in size, are hereby incorporated by reference in their entirety. ![]() The contents of the text file named “454L_008C07U_Sequence_Listing.txt”, which was created on Jan. INCORPORATION BY REFERENCE OF SEQUENCE LISTING 17, 2007, and “Methods Of Amplifying And Sequencing Nucleic Acids” filed on Jan. 10/767,894, now abandoned, “Double Ended Sequencing” filed on Jan. patent applications: “Method For Preparing Single-Stranded DNA Libraries” filed on Jan. This application also incorporates by reference the following U.S. ![]() All patent and patent applications in this paragraph are hereby fully incorporated herein by reference. 28, 2004, which claims the benefit of priority to the following applications: U.S. 31, 2007, which is a continuation of U.S. 23, 2011, which is a continuation of U.S. 14, 2012, which is a continuation of U.S. 25, 2013, now abandoned, which is a continuation of U.S. 20, 2014, which is a continuation of U.S. 23, 2016, which is a continuation of U.S. This application is a continuation of U.S. ![]()
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